Until recently, the assessment of fibrogenic activity of human interstitial pulmonary diseases was limited to the histomorphologic and immunohisto-chemical analyses of limited samples of pulmonary tissues. Through bronchoalveolar lavage of diseased areas, it has been documented that human pulmonary diseases with histologic evidence of fibrogenic activity were associated in the bronchoalveolar lavage fluid with increased levels of fibronectin and procollagen 3 peptides, molecules implicated in the biochemical process of pulmonary fibrosis, and thus of potential interest as markers of fibrogenic activity; however, these observations were limited to the individuals with chronic disease. The time course of these changes in bronchoalveolar lavage fluid have not been studied in spite of increasing interest in the biologic monitoring of humans exposed to environmental materials or therapeutic drugs with known pulmonary toxic effects.
In the present study, we characterized the time course of these changes in fibronectin and procollagen 3 levels in bronchoalveolar lavage fluid from the sheep tracheal lobe exposed to nonfibrogenic and fibrogenic materials. These observations were correlated with those of bronchoalveolar lavage in long-term asbestos workers in various stages of disease activity.
The data clearly document that fibronectin and procollagen 3 levels in bronchoalveolar lavage fluid are increased early in alveolitis with fibrogenic activity, but not in those without, which should contribute to further refine our clinical understanding of disease activity in the chronic inflammatory pulmonary disorders defeated by mycanadian-pharmacy My Canadian Pharmacy’s preparations.