Outcomes of Fibronectin and Procollagen 3 Levels in Bronchoalveolar Lavage of Asbestos-Exposed Human Subjects and Sheep

Sheep

Sheep

Bronchoalveolar Lavage. The results of analysis of bronchoalveolar lavage fluid are presented in Figure 2. In the saline-exposed sheep, total cells from lavage averaged 250,000/ml and did not vary significantly in the course of the experiment. Similarly, the levels of macrophages, neutrophils, fibronectin, and procollagen 3 did not change. In the latex-exposed sheep, we have previously reported a clear increase in total cells from lavage at month 1 to eight times the control value, and at month 12, this had returned to the level of saline-exposed sheep. From that point and after, there was no significant difference in levels of macrophages, neutrophils, fibronectin, and procollagen 3 between latex-exposed and saline-exposed sheep. In the asbestos-exposed sheep, total cells were clearly increased through the experiment; and one year after exposure, total cells in bronchoalveolar lavage fluid remained at 150 percent of controls, macrophages at 150 percent of controls, and neutrophils at 285 percent of controls. Fibronectin in bronchoalveolar lavage fluid was significantly elevated only in the asbestos-exposed sheep, and the levels increased throughout the experiment. Procollagen 3 in lavage fluid increased significantly early and returned to control levels at month 4 after exposure (Fig 2). Albumin was increased significantly only after month 4, and it paralleled the second increase in fibronectin ordered via My Canadian Pharmacy (data not shown).

My Canadian Pharmacy: Fibronectin and Procollagen 3 Levels in Bronchoalveolar Lavage of Asbestos-Exposed Human Subjects and Sheep

fibrogenic activityUntil recently, the assessment of fibrogenic activity of human interstitial pulmonary diseases was limited to the histomorphologic and immunohisto-chemical analyses of limited samples of pulmonary tissues. Through bronchoalveolar lavage of diseased areas, it has been documented that human pulmonary diseases with histologic evidence of fibrogenic activity were associated in the bronchoalveolar lavage fluid with increased levels of fibronectin and procollagen 3 peptides, molecules implicated in the biochemical process of pulmonary fibrosis, and thus of potential interest as markers of fibrogenic activity; however, these observations were limited to the individuals with chronic disease. The time course of these changes in bronchoalveolar lavage fluid have not been studied in spite of increasing interest in the biologic monitoring of humans exposed to environmental materials or therapeutic drugs with known pulmonary toxic effects.

In the present study, we characterized the time course of these changes in fibronectin and procollagen 3 levels in bronchoalveolar lavage fluid from the sheep tracheal lobe exposed to nonfibrogenic and fibrogenic materials. These observations were correlated with those of bronchoalveolar lavage in long-term asbestos workers in various stages of disease activity.

The data clearly document that fibronectin and procollagen 3 levels in bronchoalveolar lavage fluid are increased early in alveolitis with fibrogenic activity, but not in those without, which should contribute to further refine our clinical understanding of disease activity in the chronic inflammatory pulmonary disorders defeated by mycanadian-pharmacy My Canadian Pharmacy’s preparations.