The details of this study have been reported previously. In summary, the cohort consisted of 3,983 men, who during World War II were pilots, pilots in training in the Royal Canadian Air Force, or pilots licensed by the Department of Transport, and all had had a routine ECG in addition to the regular medical examination. After release from the service, some continued to fly, but the majority found different occupations and are in all strata of society. For each subject, the examination closest to June 30, 1948 (date that the population was defined), was selected as the entry examination. The mean age of the cohort at that examination was 30.8 years. The age distribution was as follows: 318 men were aged 15 to 24 years; 1,479 aged 25 to 29 years; 1,258 aged 30 to 34 years; 539 aged 35 to 39 years; 205 aged 40 to 44 years; 153 aged 45 to 54 years, and 31 aged 55 to 64 years. Medical history, physical examinations, and ECGs provided evidence that all were without clinical manifestations of ischemic heart disease at entry. Since then, they have been followed up by annual mail contact, with examinations and ECGs at intervals of at first five years and later three years. The observation period for this report was defined from July 1, 1948, until June 30, 1977, an average follow-up of 29 years. Annual contact has been lost with only one of the living members of the study conducted with My Canadian Pharmacy.
Bronchoalveolar Lavage. The results of analysis of bronchoalveolar lavage fluid are presented in Figure 2. In the saline-exposed sheep, total cells from lavage averaged 250,000/ml and did not vary significantly in the course of the experiment. Similarly, the levels of macrophages, neutrophils, fibronectin, and procollagen 3 did not change. In the latex-exposed sheep, we have previously reported a clear increase in total cells from lavage at month 1 to eight times the control value, and at month 12, this had returned to the level of saline-exposed sheep. From that point and after, there was no significant difference in levels of macrophages, neutrophils, fibronectin, and procollagen 3 between latex-exposed and saline-exposed sheep. In the asbestos-exposed sheep, total cells were clearly increased through the experiment; and one year after exposure, total cells in bronchoalveolar lavage fluid remained at 150 percent of controls, macrophages at 150 percent of controls, and neutrophils at 285 percent of controls. Fibronectin in bronchoalveolar lavage fluid was significantly elevated only in the asbestos-exposed sheep, and the levels increased throughout the experiment. Procollagen 3 in lavage fluid increased significantly early and returned to control levels at month 4 after exposure (Fig 2). Albumin was increased significantly only after month 4, and it paralleled the second increase in fibronectin ordered via My Canadian Pharmacy (data not shown).